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  • br Conclusion br In conclusion our

    2020-08-12


    5. Conclusion
    In conclusion, our results demonstrated that 8-PN induces apoptosis in HCT-116 colon cancer cells. This compound activates both the early and late stages of apoptosis as demonstrated via fluorescence micro-scopy and flow cytometry analysis. Furthermore, 8-PN may trigger both intrinsic and extrinsic apoptosis signaling pathways to cause cell death. In addition, 8-PN also induces KPT 185 arrest at G0/G1 phase in HCT-116 cells in a time-dependent manner. Given the potential anticancer effect of 8-PN, further in-depth knowledge about the molecular me-chanisms of cell death induced by 8-PN is urgently required for future research targeting colorectal cancer.
    Funding
    This study was supported by University of Malaya, Malaysia through UMRG grant No. RG336-15AFR.
    Declaration of Competing Interest
    The authors declare that they have no conflict of interest.
    References
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    M.L. Deinzer, et al., Prenylflavonoids from hops inhibit the metabolic activation of the carcinogenic heterocyclic amine 2-amino-3-methylimidazo [4, 5-f] quinoline, mediated by cDNA-expressed human CYP1A2, Drug Metab. Dispos. 28 (2000)
    [23] B.S. Reddy, A. Rivenson, Inhibitory effect KPT 185 of Bifidobacterium longum on colon, mammary, and liver carcinogenesis induced by 2-amino-3-methylimidazo [4, 5-f] quinoline, a food mutagen, Cancer Res. 53 (1993) 3914–3918. [24] P. Allsopp, S. Possemiers, D. Campbell, C. Gill, I. Rowland, A comparison of the anticancer properties of isoxanthohumol and 8-prenylnaringenin using in vitro models of colon cancer, Biofactors 39 (2013) 441–447. [25] T. Mosmann, Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays, J. Immunol. Methods 65 (1983) 55–63. [26] L. Altucci, A. Rossin, W. Raffelsberger, A. Reitmair, C. Chomienne, H. Gronemeyer, Retinoic acid-induced apoptosis in leukemia cells is mediated by paracrine action of tumor-selective death ligand TRAIL, Nat. Med. 7 (2001) 680.
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    Evaluation of the cytotoxicity, cell-cycle arrest, and apoptotic induction by Euphorbia hirta in MCF-7 breast cancer cells, Pharm. Biol. 54 (2016) 1223–1236.
    [31] S. Fani, B. Kamalidehghan, K.M. Lo, N.M. Hashim, K.M. Chow, F. Ahmadipour, Synthesis, structural characterization, and anticancer activity of a monobenzyltin compound against MCF-7 breast cancer cells, Drug Des. Devel. Ther. 9 (2015) 6191. [32] B.L. Adamsen, K.L. Kravik, O.P. Clausen, P.M. De Angelis, Apoptosis, cell cycle progression and gene expression in TP53-depleted HCT116 colon cancer cells in response to short-term 5-fluorouracil treatment, Int. J. Oncol. 31 (2007)
    [33] S.-H. Lee, X.W. Meng, K.S. Flatten, D.A. Loegering, S.H. Kaufmann, Phosphatidylserine exposure during apoptosis reflects bidirectional trafficking be-tween plasma membrane and cytoplasm, Cell Death Differ. 20 (2013) 64–76.
    Contents lists available at ScienceDirect
    Materials Science & Engineering C
    journal homepage: www.elsevier.com/locate/msec
    Antiproliferative effects of new magnetic pH-responsive drug delivery T
    system composed of Fe3O4, CaAl layered double hydroxide and levodopa on
    melanoma cancer cells
    Nahid Shahabadia,b, , Mahtab Razlansaria, Hossein Zhalehc, Kamran Mansourib
    a Inorganic Chemistry Department, Faculty of Chemistry, Razi University, Kermanshah, Iran
    b Medical Biology Research Center (MBRC), University of Medical Sciences, Kermanshah, Iran
    c Substance Abuse Prevention Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
    Keywords:
    Cell culture
    Drug release
    L-Dopa
    Layered double hydroxide
    Magnetic nano carrier
    Melanoma 
    In this study, an efficient drug delivery system composed of Fe3O4, CaAl layered double hydroxide (LDH) and L-Dopa has been synthesized through hydrogen bonds between L-Dopa and CaAl-LDH encapsulated Fe3O4 nano-particles ([email protected]@L-Dopa). The structural features of [email protected]@L-Dopa were characterized using XRD, SEM, TEM, EDX, FT-IR, VSM, TGA, XPS, zeta potential analysis and BET. All of the characterization techniques show the uniform high surface area core-shell structure with about 120 nm in average size. Also, the obtained results clearly indicate that this drug delivery system possess high potent for adsorption of L-Dopa (52 wt%) and high drug encapsulation efficiency (71%). The amount of L-Dopa release in low pHs (53.8%) which simulates the environment of cancer cells is greater than higher pHs. The in vitro cytotoxic and anticancer activities of [email protected]@L-Dopa were investigated against Mel-Rm Cells Melanoma (NCIt: C3224) using LDH colorimetric assay and differential staining cell death assay. The results showed [email protected]@L-Dopa with a lower concentration of L-Dopa, illustrate a higher cytotoxicity and anticancer activity.