br Conclusion br In conclusion our
In conclusion, our results demonstrated that 8-PN induces apoptosis in HCT-116 colon cancer cells. This compound activates both the early and late stages of apoptosis as demonstrated via fluorescence micro-scopy and flow cytometry analysis. Furthermore, 8-PN may trigger both intrinsic and extrinsic apoptosis signaling pathways to cause cell death. In addition, 8-PN also induces KPT 185 arrest at G0/G1 phase in HCT-116 cells in a time-dependent manner. Given the potential anticancer eﬀect of 8-PN, further in-depth knowledge about the molecular me-chanisms of cell death induced by 8-PN is urgently required for future research targeting colorectal cancer.
This study was supported by University of Malaya, Malaysia through UMRG grant No. RG336-15AFR.
Declaration of Competing Interest
The authors declare that they have no conflict of interest.
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Materials Science & Engineering C
journal homepage: www.elsevier.com/locate/msec
Antiproliferative eﬀects of new magnetic pH-responsive drug delivery T
system composed of Fe3O4, CaAl layered double hydroxide and levodopa on
melanoma cancer cells
Nahid Shahabadia,b, , Mahtab Razlansaria, Hossein Zhalehc, Kamran Mansourib
a Inorganic Chemistry Department, Faculty of Chemistry, Razi University, Kermanshah, Iran
b Medical Biology Research Center (MBRC), University of Medical Sciences, Kermanshah, Iran
c Substance Abuse Prevention Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
Layered double hydroxide
Magnetic nano carrier
In this study, an eﬃcient drug delivery system composed of Fe3O4, CaAl layered double hydroxide (LDH) and L-Dopa has been synthesized through hydrogen bonds between L-Dopa and CaAl-LDH encapsulated Fe3O4 nano-particles ([email protected]@L-Dopa). The structural features of [email protected]@L-Dopa were characterized using XRD, SEM, TEM, EDX, FT-IR, VSM, TGA, XPS, zeta potential analysis and BET. All of the characterization techniques show the uniform high surface area core-shell structure with about 120 nm in average size. Also, the obtained results clearly indicate that this drug delivery system possess high potent for adsorption of L-Dopa (52 wt%) and high drug encapsulation eﬃciency (71%). The amount of L-Dopa release in low pHs (53.8%) which simulates the environment of cancer cells is greater than higher pHs. The in vitro cytotoxic and anticancer activities of [email protected]@L-Dopa were investigated against Mel-Rm Cells Melanoma (NCIt: C3224) using LDH colorimetric assay and diﬀerential staining cell death assay. The results showed [email protected]@L-Dopa with a lower concentration of L-Dopa, illustrate a higher cytotoxicity and anticancer activity.